anti il 12p70  (R&D Systems)

Journal: Nature Communications

Article Title: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells

doi: 10.1038/s41467-024-54420-w

Figure Lengend Snippet: Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).

Article Snippet: For IL-12p70 and CD40L blocking experiments, anti-Human Il-12p70 (R&D Systems, cat #MAB219-100, 10 μg/ml final), anti-CD40 (BioLegend, cat #313019, clone HB14, 10 μg/ml final) or isotype control mouse IgG1 (BioLegend, cat #400166, clone MOPC21, 10 μg/ml final) were added in the corresponding wells.

Techniques: Expressing, Control, Inhibition